To improve normality, every ratio investigation was basically switched before analyses
A standardized, collective index out of cross-physical fitness try computed for each mixture of maternal tree and you will pollen donor, predicated on mature good fresh fruit put, seed products germination, and you will survivorship and you will growth of seedlings die besten russischen Dating-Seiten. For every single varieties, mixed-model study away from difference was used to evaluate the results from crossing cures (fixed feeling; having maternal forest incorporated since an arbitrary impression) for the part of give-pollinated plant life form adult fresh fruit, prices regarding vegetables germination and you will seedling survivorship, seedling size on step one yr, and you may collective physical fitness. Numerous habits was basically checked out having fun with ANOVA: (a) in addition to every solutions, (b) leaving out imbalanced treatments, allowing investigations out of communications terms, (c) without selfing cures (once the maternal woods was basically mostly otherwise totally thinking-incompatible), and you will (d) grouping all the contained in this-Sinharaja outcrossing services to evaluate the end result regarding contained in this- vs. between-forest crossing. The end result from crossing range on each factor is next examined playing with linear or quadratic regression data, with regards to the form of the partnership. Lastly, per maternal forest, the effects regarding nearest-next-door neighbor and you can long-length mating was estimated using indicator out of biparental inbreeding anxiety and outbreeding anxiety, respectively, considering collective exercise beliefs.
Syzygium rubicundum
Fruit abortion was heavy for all trees, resulting in low fruit set (range across treatments: 2.0–9.7%; Fig. 2a). The timing of abortion was not discernable across treatments. Self-compatibility was low, but variable, across maternal trees (Fig. 2a). Flowers used for tests of apomixis (N = 360) and autogamy (N = 582) failed to set fruit. All analyses of variance in fruit set revealed a highly significant treatment effect and significant maternal tree effect, but no significant interaction between treatment and maternal tree (Tables 2A and 3A). For all three trees, the percentage of experimental flowers setting mature fruit showed a consistent increase with crossing distance, followed by a severe decline in fruit set with the distant between-forest treatment (Fig. 2a). The relationship between crossing distance and fruit set was nearly identical for the three maternal trees and significant with or without the self-pollinated treatment included in the model (quadratic regression model: arcsine square-root [fruit set] = crossing distance [km] + crossing distance 2 ; results without self-pollinated treatment: F2,57 = 8.25, P < 0.0007, R 2 = 0.47). Peak mean fruit set occurred at a crossing distance of 1–2 km (distant within-forest treatment) and was 1.7–4.7 times greater than mean fruit set rates for other hand-pollination treatments, averaged across maternal trees. Mean fruit set rate for the distant within-forest treatment was significantly greater than those for all treatments except distant-neighbor and open-pollinated, but consistently exceeded fruit set of open-pollinated flowers (Fig. 2a).
Shorea cordifolia
Fruit set was also low for Sh. cordifolia (range across treatments: 0–5.3%; Fig. 2b). Again, the timing of fruit abortion was not discernable among treatments. Selfed and distant between-forest treatments resulted in 0% and <1% fruit set, respectively. Fruit set from the intermediate-distance cross-pollinations varied across maternal trees, but with one exception (nearest-neighbor treatment at Tree number 1) indicated optimal fruit set at an outcrossing range of ?2 km (distant neighbor treatment; Fig. 2b). All analyses of variance in fruit set revealed a highly significant treatment effect, but no maternal tree effect (Tables 2B and 3B). The relationship between crossing distance and fruit set was significant only when the selfed treatment was excluded (quadratic regression model: arcsin square root [fruit set] = crossing distance [km] + crossing distance 2 ; Fdos,57 = 5.71, P < 0.006, R 2 = 0.41). At each maternal tree, fruit set rate for open-pollinated flowers was greater than that for all hand-cross treatments, suggesting that some aspect of the hand-pollination procedure (e.g., flower handling, bagging) caused reduced fruit set in Sh. cordifolia.